Composite

Part:BBa_K209436:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)

pEF1-SSFYFP-hM2D-Vav


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
    Illegal PstI site found at 2922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1161
    Illegal PstI site found at 346
    Illegal PstI site found at 2922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BglII site found at 2588
    Illegal BglII site found at 4544
    Illegal BamHI site found at 955
    Illegal BamHI site found at 1665
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
    Illegal PstI site found at 2922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal PstI site found at 2922
    Illegal NgoMIV site found at 3275
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 3452
    Illegal BsaI.rc site found at 3646
    Illegal BsaI.rc site found at 4460
    Illegal SapI site found at 2349


Design Notes

Cloned into pEF5/FRT/V5-DEST.

Source

References